Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3637402..3638021 Replicon chromosome
Accession NZ_CP024834
Organism Klebsiella pneumoniae strain CRKP-2297

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CU075_RS18380 Protein ID WP_002892050.1
Coordinates 3637803..3638021 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CU075_RS18375 Protein ID WP_002892066.1
Coordinates 3637402..3637776 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU075_RS18365 3632554..3633747 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CU075_RS18370 3633770..3636916 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CU075_RS18375 3637402..3637776 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CU075_RS18380 3637803..3638021 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CU075_RS18385 3638184..3638750 + 567 WP_002892030.1 maltose O-acetyltransferase -
CU075_RS29735 3638722..3638862 - 141 WP_004147370.1 hypothetical protein -
CU075_RS18390 3638883..3639353 + 471 WP_020802585.1 YlaC family protein -
CU075_RS18395 3639328..3640779 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
CU075_RS18400 3640880..3641578 + 699 WP_023287311.1 GNAT family N-acetyltransferase -
CU075_RS18405 3641575..3641715 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CU075_RS18410 3641715..3641978 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T88759 WP_002892050.1 NZ_CP024834:3637803-3638021 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T88759 NZ_CP024834:3637803-3638021 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT88759 WP_002892066.1 NZ_CP024834:3637402-3637776 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT88759 NZ_CP024834:3637402-3637776 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References