Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1036291..1037027 Replicon chromosome
Accession NZ_CP024834
Organism Klebsiella pneumoniae strain CRKP-2297

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag CU075_RS05365 Protein ID WP_032433360.1
Coordinates 1036545..1037027 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CU075_RS05360 Protein ID WP_003026799.1
Coordinates 1036291..1036557 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU075_RS05335 1031937..1033076 + 1140 WP_032433368.1 mannitol dehydrogenase -
CU075_RS05340 1033105..1033767 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
CU075_RS05345 1033751..1034755 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
CU075_RS05350 1034773..1035405 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
CU075_RS05355 1035415..1035978 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
CU075_RS05360 1036291..1036557 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CU075_RS05365 1036545..1037027 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
CU075_RS05370 1037388..1037987 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
CU075_RS05375 1038200..1039144 - 945 WP_077254249.1 fimbrial protein -
CU075_RS05380 1039156..1039734 - 579 WP_032432061.1 type 1 fimbrial protein -
CU075_RS05385 1039738..1040478 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1006514..1047970 41456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T88749 WP_032433360.1 NZ_CP024834:1036545-1037027 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T88749 NZ_CP024834:1036545-1037027 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT88749 WP_003026799.1 NZ_CP024834:1036291-1036557 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT88749 NZ_CP024834:1036291-1036557 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References