Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 181834..182477 Replicon plasmid pCREC-532_1
Accession NZ_CP024831
Organism Escherichia coli strain CREC-532

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag CU076_RS26150 Protein ID WP_001044768.1
Coordinates 182061..182477 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag CU076_RS26145 Protein ID WP_001261287.1
Coordinates 181834..182064 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU076_RS26130 177012..178145 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
CU076_RS26135 178408..181527 - 3120 WP_001553851.1 hypothetical protein -
CU076_RS26145 181834..182064 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CU076_RS26150 182061..182477 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
CU076_RS26155 182639..184777 - 2139 WP_000350635.1 AAA family ATPase -
CU076_RS26160 185242..186237 + 996 WP_000246636.1 hypothetical protein -
CU076_RS26165 186241..187173 + 933 WP_000991832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / erm(B) / tet(B) / aac(3)-IId / blaTEM-1B - 1..216181 216181
- flank IS/Tn - - 187310..187813 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T88744 WP_001044768.1 NZ_CP024831:182061-182477 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T88744 NZ_CP024831:182061-182477 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT88744 WP_001261287.1 NZ_CP024831:181834-182064 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT88744 NZ_CP024831:181834-182064 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References