Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 32937..33496 Replicon plasmid pCREC-532_1
Accession NZ_CP024831
Organism Escherichia coli strain CREC-532

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CU076_RS25100 Protein ID WP_000421272.1
Coordinates 32937..33212 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CU076_RS25105 Protein ID WP_001387467.1
Coordinates 33212..33496 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU076_RS25060 28987..29727 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
CU076_RS25065 29848..30030 - 183 WP_042065278.1 hypothetical protein -
CU076_RS25075 30373..30680 - 308 Protein_31 type II toxin-antitoxin system ParD family antitoxin -
CU076_RS25085 31173..32342 + 1170 WP_001072355.1 hypothetical protein -
CU076_RS25090 32375..32503 + 129 Protein_33 IS481 family transposase -
CU076_RS25095 32538..32831 - 294 WP_001105060.1 hypothetical protein -
CU076_RS25100 32937..33212 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CU076_RS25105 33212..33496 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CU076_RS25110 34101..34853 + 753 WP_000562172.1 IclR family transcriptional regulator -
CU076_RS25115 34899..35864 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CU076_RS25120 35897..36277 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
CU076_RS25125 36302..36691 - 390 Protein_40 dihydrodipicolinate synthase family protein -
CU076_RS25130 36757..37461 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
CU076_RS27865 37512..37769 - 258 Protein_42 DUF3330 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / erm(B) / tet(B) / aac(3)-IId / blaTEM-1B - 1..216181 216181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T88738 WP_000421272.1 NZ_CP024831:c33212-32937 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T88738 NZ_CP024831:c33212-32937 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT88738 WP_001387467.1 NZ_CP024831:c33496-33212 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT88738 NZ_CP024831:c33496-33212 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References