Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 18193..18794 Replicon plasmid pCREC-532_1
Accession NZ_CP024831
Organism Escherichia coli strain CREC-532

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CU076_RS24975 Protein ID WP_001216034.1
Coordinates 18414..18794 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CU076_RS24970 Protein ID WP_001190712.1
Coordinates 18193..18414 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU076_RS24960 15186..16457 - 1272 WP_048266692.1 restriction endonuclease subunit S -
CU076_RS24965 16454..18010 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
CU076_RS24970 18193..18414 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CU076_RS24975 18414..18794 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CU076_RS24980 18799..18978 + 180 WP_001513661.1 hypothetical protein -
CU076_RS24985 19006..19365 + 360 WP_001513660.1 hypothetical protein -
CU076_RS24990 19289..19702 + 414 Protein_17 DDE-type integrase/transposase/recombinase -
CU076_RS24995 19652..19969 - 318 WP_001513659.1 hypothetical protein -
CU076_RS25000 20197..21177 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
CU076_RS25005 21421..22824 + 1404 WP_001373486.1 S-methylmethionine permease -
CU076_RS25010 22811..23743 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / erm(B) / tet(B) / aac(3)-IId / blaTEM-1B - 1..216181 216181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T88737 WP_001216034.1 NZ_CP024831:18414-18794 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T88737 NZ_CP024831:18414-18794 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT88737 WP_001190712.1 NZ_CP024831:18193-18414 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT88737 NZ_CP024831:18193-18414 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References