Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3873043..3873874 Replicon chromosome
Accession NZ_CP024830
Organism Escherichia coli strain CREC-532

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag CU076_RS20220 Protein ID WP_000854814.1
Coordinates 3873500..3873874 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag CU076_RS20215 Protein ID WP_001285585.1
Coordinates 3873043..3873411 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU076_RS20175 3868275..3869345 + 1071 WP_000102643.1 patatin-like phospholipase family protein -
CU076_RS20180 3869481..3870164 + 684 WP_000775500.1 hypothetical protein -
CU076_RS20185 3870180..3870590 + 411 WP_000846713.1 hypothetical protein -
CU076_RS20195 3870811..3871632 + 822 WP_001234530.1 DUF945 domain-containing protein -
CU076_RS20200 3871714..3872193 + 480 WP_000860076.1 antirestriction protein -
CU076_RS20205 3872209..3872685 + 477 WP_001186773.1 RadC family protein -
CU076_RS20210 3872748..3872969 + 222 WP_000692323.1 DUF987 domain-containing protein -
CU076_RS20215 3873043..3873411 + 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
CU076_RS20220 3873500..3873874 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
CU076_RS20225 3873871..3874065 + 195 WP_000988600.1 hypothetical protein -
CU076_RS27525 3874078..3874191 + 114 WP_001161660.1 DUF957 domain-containing protein -
CU076_RS20230 3874480..3874626 - 147 Protein_3675 transposase domain-containing protein -
CU076_RS20235 3874680..3874862 + 183 WP_001016348.1 hypothetical protein -
CU076_RS20240 3874963..3875292 - 330 WP_000450409.1 DUF496 family protein -
CU076_RS20245 3875464..3876522 - 1059 WP_001200905.1 FUSC family protein -
CU076_RS20250 3876720..3877193 - 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
CU076_RS20255 3877312..3878478 - 1167 WP_000830163.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T88732 WP_000854814.1 NZ_CP024830:3873500-3873874 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T88732 NZ_CP024830:3873500-3873874 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT88732 WP_001285585.1 NZ_CP024830:3873043-3873411 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT88732 NZ_CP024830:3873043-3873411 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References