Detailed information of TA system TA088721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3256560..3257198
• Replicon: chromosome
• Accession: NZ_CP024830
• Organism: Escherichia coli strain CREC-532

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: CU076_RS16825
• Protein ID: WP_001447010.1
• Coordinates: 3256560..3256736 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CU076_RS16830
• Protein ID: WP_001270286.1
• Coordinates: 3256782..3257198 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU076_RS16805	3252179..3253393	-	1215	WP_001326689.1	BenE family transporter YdcO	-
CU076_RS16810	3253446..3253982	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
CU076_RS16815	3254055..3256016	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CU076_RS16820	3256108..3256338	-	231	WP_000494244.1	YncJ family protein	-
CU076_RS16825	3256560..3256736	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CU076_RS16830	3256782..3257198	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CU076_RS16835	3257277..3258683	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
CU076_RS16840	3258928..3260073	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
CU076_RS16845	3260091..3261104	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
CU076_RS16850	3261105..3262046	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T88721 WP_001447010.1 NZ_CP024830:3256560-3256736 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T88721 NZ_CP024830:3256560-3256736 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT88721 WP_001270286.1 NZ_CP024830:3256782-3257198 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT88721 NZ_CP024830:3256782-3257198 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8