Detailed information of TA system TA088719

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2023406..2024100
• Replicon: chromosome
• Accession: NZ_CP024830
• Organism: Escherichia coli strain CREC-532

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: CU076_RS10370
• Protein ID: WP_001094398.1
• Coordinates: 2023732..2024100 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CU076_RS10365
• Protein ID: WP_077249034.1
• Coordinates: 2023406..2023711 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU076_RS10325	2019356..2019811	+	456	WP_000581506.1	hypothetical protein	-
CU076_RS27365	2019912..2020120	+	209	Protein_1878	DUF905 domain-containing protein	-
CU076_RS10335	2020221..2021042	+	822	WP_001234565.1	DUF945 domain-containing protein	-
CU076_RS10345	2021384..2021857	+	474	WP_000855059.1	antirestriction protein	-
CU076_RS10350	2021873..2022349	+	477	WP_001424026.1	RadC family protein	-
CU076_RS10355	2022418..2022639	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CU076_RS10360	2022658..2023302	+	645	WP_000086755.1	hypothetical protein	-
CU076_RS10365	2023406..2023711	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CU076_RS10370	2023732..2024100	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
CU076_RS10375	2024421..2024759	-	339	Protein_1886	LysR family transcriptional regulator	-
CU076_RS10380	2024810..2025766	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
CU076_RS10385	2025776..2027974	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
CU076_RS10390	2027971..2028927	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T88719 WP_001094398.1 NZ_CP024830:2023732-2024100 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T88719 NZ_CP024830:2023732-2024100 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT88719 WP_077249034.1 NZ_CP024830:2023406-2023711 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT88719 NZ_CP024830:2023406-2023711 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4