Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4616406..4617037 Replicon chromosome
Accession NZ_CP024826
Organism Escherichia coli strain CREC-544

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag CU077_RS23380 Protein ID WP_001260301.1
Coordinates 4616762..4617037 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CU077_RS23375 Protein ID WP_000593555.1
Coordinates 4616406..4616765 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU077_RS23350 4612539..4613483 + 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -
CU077_RS23355 4613480..4614313 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CU077_RS23360 4614313..4615077 + 765 WP_001136211.1 nickel import ATP-binding protein NikD -
CU077_RS23365 4615074..4615880 + 807 WP_061092823.1 nickel import ATP-binding protein NikE -
CU077_RS23370 4615885..4616286 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CU077_RS23375 4616406..4616765 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CU077_RS23380 4616762..4617037 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
CU077_RS23385 4617110..4618234 - 1125 WP_001314210.1 ABC transporter permease -
CU077_RS23390 4618234..4620969 - 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
CU077_RS23395 4620966..4622033 - 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T88697 WP_001260301.1 NZ_CP024826:c4617037-4616762 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T88697 NZ_CP024826:c4617037-4616762 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT88697 WP_000593555.1 NZ_CP024826:c4616765-4616406 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT88697 NZ_CP024826:c4616765-4616406 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References