Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 760866..761442 Replicon chromosome
Accession NZ_CP024826
Organism Escherichia coli strain CREC-544

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag CU077_RS03780 Protein ID WP_001131965.1
Coordinates 760866..761153 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag CU077_RS03785 Protein ID WP_000063158.1
Coordinates 761140..761442 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU077_RS03755 756275..757687 - 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -
CU077_RS03760 757864..758028 + 165 WP_000394263.1 DUF1127 domain-containing protein -
CU077_RS03765 758070..758408 - 339 WP_000132633.1 endoribonuclease SymE -
CU077_RS03770 758623..759291 - 669 Protein_674 DUF3387 domain-containing protein -
CU077_RS26110 759285..759458 - 174 Protein_675 SAM-dependent DNA methyltransferase -
CU077_RS03775 759503..760669 - 1167 WP_000800823.1 restriction endonuclease -
CU077_RS03780 760866..761153 + 288 WP_001131965.1 BrnT family toxin Toxin
CU077_RS03785 761140..761442 + 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
CU077_RS03790 761476..762432 - 957 WP_001361361.1 GTPase -
CU077_RS03795 762443..762646 - 204 WP_000467859.1 YbdD/YjiX family protein -
CU077_RS03800 762696..764846 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
CU077_RS03810 765114..766334 - 1221 WP_000873756.1 amino acid deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T88678 WP_001131965.1 NZ_CP024826:760866-761153 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T88678 NZ_CP024826:760866-761153 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT88678 WP_000063158.1 NZ_CP024826:761140-761442 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT88678 NZ_CP024826:761140-761442 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References