88660

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 8752..9245 Replicon plasmid pCREC-591_1
Accession NZ_CP024822
Organism Escherichia coli strain CREC-591

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag CU078_RS26895 Protein ID WP_001230709.1
Coordinates 8991..9245 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag CU078_RS26890 Protein ID WP_000151456.1
Coordinates 8752..8994 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU078_RS26865 4158..5420 + 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
CU078_RS26870 5638..6837 - 1200 WP_000948429.1 IS91 family transposase -
CU078_RS26875 6847..7035 - 189 WP_000957857.1 hypothetical protein -
CU078_RS26880 7361..8236 + 876 WP_031942271.1 class A extended-spectrum beta-lactamase CTX-M-123 -
CU078_RS26885 8283..8615 - 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
CU078_RS26890 8752..8994 - 243 WP_000151456.1 hypothetical protein Antitoxin
CU078_RS26895 8991..9245 - 255 WP_001230709.1 hypothetical protein Toxin
CU078_RS26900 9242..9784 - 543 WP_001083904.1 hypothetical protein -
CU078_RS26905 9888..10403 - 516 WP_001025393.1 J domain-containing protein -
CU078_RS26915 10807..11136 - 330 WP_000855119.1 hypothetical protein -
CU078_RS26920 11951..12364 + 414 WP_000465036.1 hypothetical protein -
CU078_RS26925 12366..13145 + 780 WP_001164198.1 site-specific integrase -
CU078_RS26930 13325..13969 + 645 WP_001144036.1 ParA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-123 / mph(A) / fosA3 - 1..118156 118156


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)

Antitoxin

Toxin YoeB

(5-84)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T88660 WP_001230709.1 NZ_CP024822:c9245-8991 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T88660 NZ_CP024822:c9245-8991 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT88660 WP_000151456.1 NZ_CP024822:c8994-8752 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT88660 NZ_CP024822:c8994-8752 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References