Detailed information of TA system TA088657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4441616..4442241
• Replicon: chromosome
• Accession: NZ_CP024821
• Organism: Escherichia coli strain CREC-591

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CU078_RS23380
• Protein ID: WP_000911330.1
• Coordinates: 4441843..4442241 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CU078_RS23375
• Protein ID: WP_000450524.1
• Coordinates: 4441616..4441843 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU078_RS23350	4437419..4437889	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CU078_RS23355	4437889..4438461	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CU078_RS23360	4438607..4439485	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CU078_RS23365	4439502..4440536	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
CU078_RS23370	4440749..4441462	+	714	WP_001347624.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CU078_RS23375	4441616..4441843	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CU078_RS23380	4441843..4442241	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CU078_RS23385	4442388..4443251	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
CU078_RS23390	4443266..4445281	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
CU078_RS23395	4445355..4446053	+	699	WP_000679812.1	esterase	-
CU078_RS23400	4446134..4446334	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T88657 WP_000911330.1 NZ_CP024821:4441843-4442241 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T88657 NZ_CP024821:4441843-4442241 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT88657 WP_000450524.1 NZ_CP024821:4441616-4441843 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT88657 NZ_CP024821:4441616-4441843 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33