Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 578172..578810 Replicon chromosome
Accession NZ_CP024821
Organism Escherichia coli strain CREC-591

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CU078_RS03170 Protein ID WP_000813794.1
Coordinates 578634..578810 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CU078_RS03165 Protein ID WP_001270286.1
Coordinates 578172..578588 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU078_RS03145 573324..574265 - 942 WP_001251315.1 ABC transporter permease -
CU078_RS03150 574266..575279 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
CU078_RS03155 575297..576442 - 1146 WP_072644797.1 ABC transporter substrate-binding protein -
CU078_RS03160 576687..578093 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
CU078_RS03165 578172..578588 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CU078_RS03170 578634..578810 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CU078_RS03175 579032..579262 + 231 WP_000494244.1 YncJ family protein -
CU078_RS03180 579354..581315 - 1962 WP_072644798.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CU078_RS03185 581388..581924 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CU078_RS03190 581977..583191 + 1215 WP_001348055.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 583231..584379 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T88644 WP_000813794.1 NZ_CP024821:c578810-578634 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T88644 NZ_CP024821:c578810-578634 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT88644 WP_001270286.1 NZ_CP024821:c578588-578172 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT88644 NZ_CP024821:c578588-578172 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References