Detailed information of TA system TA088634

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 5079663..5080317
• Replicon: chromosome
• Accession: NZ_CP024819
• Organism: Citrobacter freundii strain CRCB-101

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: CU079_RS24635
• Protein ID: WP_043017506.1
• Coordinates: 5079663..5080070 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: CU079_RS24640
• Protein ID: WP_016154349.1
• Coordinates: 5080051..5080317 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU079_RS24615	5075556..5077289	-	1734	WP_100194818.1	single-stranded-DNA-specific exonuclease RecJ	-
CU079_RS24620	5077295..5078008	-	714	WP_100194819.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
CU079_RS24625	5078032..5078928	-	897	WP_003825519.1	site-specific tyrosine recombinase XerD	-
CU079_RS24630	5079042..5079563	+	522	WP_100194820.1	flavodoxin FldB	-
CU079_RS24635	5079663..5080070	-	408	WP_043017506.1	protein YgfX	Toxin
CU079_RS24640	5080051..5080317	-	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
CU079_RS24650	5080574..5081554	+	981	WP_043017507.1	tRNA-modifying protein YgfZ	-
CU079_RS24655	5081646..5082305	-	660	WP_043017508.1	hemolysin III family protein	-
CU079_RS24660	5082469..5082780	-	312	WP_043017509.1	N(4)-acetylcytidine aminohydrolase	-
CU079_RS24665	5082833..5083561	+	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-
CU079_RS24670	5083682..5085115	+	1434	WP_100194821.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15891.76 Da
• Isoelectric Point: 11.5692

>T88634 WP_043017506.1 NZ_CP024819:c5080070-5079663 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMNGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDVGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T88634 NZ_CP024819:c5080070-5079663 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGCTGCTCATTCATGGGCTGGTGGCGGT
ATTCATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TCCGAAGCCAGCGCCGAATTAACGCCTGTCAGGGCGAGATTCGGCTGTTGATGAACGGTCGACTGCGCTGGCAAGGGCAA
GAGTGGTCTATTGTTAGCGCACCCTGGATGGTCAAAACGGGCATGATGCTGCGTTTGCGTTCTGATGTCGGAAAGCGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTGCGACGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT88634 WP_016154349.1 NZ_CP024819:c5080317-5080051 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT88634 NZ_CP024819:c5080317-5080051 [Citrobacter freundii]
ATGGATATTAACAACAAAGCGCGTATTCATTGGGCATGCCGTCGCGGTATGCGCGAGTTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATACGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TGTTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTCAGTTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: R8WMJ6