Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3298787..3299426 Replicon chromosome
Accession NZ_CP024819
Organism Citrobacter freundii strain CRCB-101

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag CU079_RS16015 Protein ID WP_043016254.1
Coordinates 3298787..3298963 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CU079_RS16020 Protein ID WP_155762299.1
Coordinates 3299010..3299426 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU079_RS15995 3295565..3295795 - 231 WP_100194363.1 DUF2554 family protein -
CU079_RS16000 3295985..3296110 - 126 WP_003020230.1 DUF2474 domain-containing protein -
CU079_RS16005 3296110..3297120 - 1011 WP_043016252.1 cytochrome d ubiquinol oxidase subunit II -
CU079_RS16010 3297120..3298523 - 1404 WP_080625215.1 cytochrome ubiquinol oxidase subunit I -
CU079_RS16015 3298787..3298963 + 177 WP_043016254.1 type II toxin-antitoxin system HicA family toxin Toxin
CU079_RS16020 3299010..3299426 + 417 WP_155762299.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CU079_RS16025 3299519..3300928 + 1410 WP_075146200.1 PLP-dependent aminotransferase family protein -
CU079_RS16030 3301258..3302403 + 1146 WP_080625212.1 ABC transporter substrate-binding protein -
CU079_RS16035 3302420..3303436 + 1017 WP_100194364.1 ABC transporter ATP-binding protein -
CU079_RS16040 3303437..3304381 + 945 WP_043016259.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-56)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6768.73 Da        Isoelectric Point: 11.2510

>T88624 WP_043016254.1 NZ_CP024819:3298787-3298963 [Citrobacter freundii]
VKQSEFRRWLESQGVEVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIQKQPGLQ

Download         Length: 177 bp

>T88624 NZ_CP024819:3298787-3298963 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTTGAAGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAGATAAAAGAGGCTCTGCGAAAAGCGATCCAAAAGC
AGCCAGGCTTACAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15032.29 Da        Isoelectric Point: 4.5468

>AT88624 WP_155762299.1 NZ_CP024819:3299010-3299426 [Citrobacter freundii]
MRYPVNLVPAVEGGFVVSFPDIPEALTQGETRHDALQAAQAALVTAFEFYFDDNEPIPLPSAVVAGADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSLTML

Download         Length: 417 bp

>AT88624 NZ_CP024819:3299010-3299426 [Citrobacter freundii]
ATGCGTTATCCCGTTAATCTAGTACCCGCCGTGGAAGGTGGGTTTGTGGTGTCTTTTCCGGATATTCCTGAAGCATTAAC
TCAGGGAGAAACCCGTCATGATGCTTTGCAGGCTGCGCAAGCCGCATTGGTCACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTGCCTTCGGCGGTTGTCGCTGGCGCTGACTATGTGGAAATACCACTGAGCGTTGCGTCTAAAGTT
CTGTTGTTAAATGCCTTCCTTGAATCGAATATTACCCAGCAGGAACTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
CACTCGCTTATTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAGATAGCTGCCCGCGCCTTAGGCAAAGAACTGT
CATTAACGATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References