Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2026605..2027225 Replicon chromosome
Accession NZ_CP024819
Organism Citrobacter freundii strain CRCB-101

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CU079_RS09835 Protein ID WP_002892050.1
Coordinates 2026605..2026823 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag CU079_RS09840 Protein ID WP_003021733.1
Coordinates 2026851..2027225 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU079_RS09800 2021963..2022592 + 630 WP_043015412.1 membrane protein -
CU079_RS09805 2022657..2023010 + 354 WP_003831033.1 DUF1428 family protein -
CU079_RS09810 2023062..2024615 - 1554 WP_100194051.1 EAL domain-containing protein -
CU079_RS09815 2024804..2025064 + 261 WP_043015414.1 type B 50S ribosomal protein L31 -
CU079_RS09820 2025066..2025206 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
CU079_RS09825 2025286..2025756 - 471 WP_043015415.1 YlaC family protein -
CU079_RS09830 2025873..2026424 - 552 WP_043015416.1 maltose O-acetyltransferase -
CU079_RS09835 2026605..2026823 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CU079_RS09840 2026851..2027225 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
CU079_RS09845 2027714..2030863 - 3150 WP_043015417.1 multidrug efflux RND transporter permease subunit -
CU079_RS09850 2030886..2032079 - 1194 WP_100194052.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T88623 WP_002892050.1 NZ_CP024819:c2026823-2026605 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T88623 NZ_CP024819:c2026823-2026605 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT88623 WP_003021733.1 NZ_CP024819:c2027225-2026851 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT88623 NZ_CP024819:c2027225-2026851 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGTCT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References