Detailed information of TA system TA088622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1426028..1426604
• Replicon: chromosome
• Accession: NZ_CP024819
• Organism: Citrobacter freundii strain CRCB-101

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: CU079_RS07005
• Protein ID: WP_075145248.1
• Coordinates: 1426028..1426315 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CU079_RS07010
• Protein ID: WP_043018311.1
• Coordinates: 1426302..1426604 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU079_RS06980	1421382..1422794	-	1413	WP_043018306.1	PLP-dependent aminotransferase family protein	-
CU079_RS06985	1422972..1423136	+	165	WP_008786105.1	DUF1127 domain-containing protein	-
CU079_RS06990	1423282..1423746	+	465	WP_043018307.1	MarR family transcriptional regulator	-
CU079_RS06995	1423753..1424820	+	1068	WP_075145249.1	HlyD family secretion protein	-
CU079_RS07000	1424810..1425874	+	1065	WP_043018309.1	DUF2955 domain-containing protein	-
CU079_RS07005	1426028..1426315	+	288	WP_075145248.1	BrnT family toxin	Toxin
CU079_RS07010	1426302..1426604	+	303	WP_043018311.1	BrnA antitoxin family protein	Antitoxin
CU079_RS07015	1426717..1427673	-	957	WP_043018312.1	GTPase	-
CU079_RS07020	1427686..1427880	-	195	WP_003830038.1	YbdD/YjiX family protein	-
CU079_RS07025	1427995..1430145	-	2151	WP_087052214.1	pyruvate/proton symporter BtsT	-
CU079_RS07030	1430435..1430977	-	543	WP_003830032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11219.68 Da
• Isoelectric Point: 8.5380

>T88622 WP_075145248.1 NZ_CP024819:1426028-1426315 [Citrobacter freundii]
MPMEFVWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGMMYGVVVILVAHSVRFESGVEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T88622 NZ_CP024819:1426028-1426315 [Citrobacter freundii]
ATGCCAATGGAGTTTGTATGGGATGCAAACAAGGCACAAAGTAACCACCGTAAACATGGTGTTCGCTTTGAGGACGCAGT
ATTGGTTTTCGACGATCCCCGGCATTTGTCCAGACAGGATCGTTATGAGAACGGTGAGTATCGATGGCAGACCATAGGGA
TGATGTATGGTGTGGTGGTCATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCGTTGAAGTCATTCGTATCATCAGT
GCCAGAAAGGCAGACAGTAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11485.10 Da
• Isoelectric Point: 10.1293

>AT88622 WP_043018311.1 NZ_CP024819:1426302-1426604 [Citrobacter freundii]
MSMVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEANRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT88622 NZ_CP024819:1426302-1426604 [Citrobacter freundii]
ATGAGCATGGTTAAACATAAACGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
TAAACCGGATGATGATATTGATTACAGTGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCTAATCGCGGTAAGT
TTTTCCGTCCGTTGAAAACGCAGGCATCGGTGCGAATTGATGCCGATGTGATGGAGTGGCTAAAGCGCCCCGGGAAGGGT
TACCAGACGCGGCTGAATGCTATTTTGCGCGAGGCTATGTTGAGAGAGCAAAACAAGAAATAG