Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 1019703..1020291 Replicon chromosome
Accession NZ_CP024819
Organism Citrobacter freundii strain CRCB-101

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag CU079_RS04990 Protein ID WP_100193831.1
Coordinates 1019974..1020291 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag CU079_RS04985 Protein ID WP_100193830.1
Coordinates 1019703..1019981 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU079_RS04975 1017158..1018789 + 1632 WP_047407292.1 Na/Pi cotransporter family protein -
CU079_RS04980 1018905..1019594 - 690 WP_100193829.1 dipeptidase PepE -
CU079_RS04985 1019703..1019981 - 279 WP_100193830.1 putative addiction module antidote protein Antitoxin
CU079_RS04990 1019974..1020291 - 318 WP_100193831.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CU079_RS04995 1020503..1021021 - 519 WP_100193832.1 GNAT family N-acetyltransferase -
CU079_RS05000 1021048..1021509 - 462 WP_100193833.1 GNAT family N-acetyltransferase -
CU079_RS05005 1021571..1022173 - 603 WP_100193834.1 helix-turn-helix domain-containing protein -
CU079_RS05010 1022217..1022912 + 696 WP_100193835.1 B3/4 domain-containing protein -
CU079_RS05015 1022933..1023826 + 894 WP_100193836.1 dihydrodipicolinate synthase family protein -
CU079_RS05020 1023887..1025098 + 1212 WP_100193837.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(2-96)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12035.04 Da        Isoelectric Point: 9.5858

>T88620 WP_100193831.1 NZ_CP024819:c1020291-1019974 [Citrobacter freundii]
IMEILQTMVFQRWEHNLRDRRAKTLIATRLFRLANGLAGDVKPVGEGVSEMRISYGPGYRIYFKQQGCRIIILLCGGDKS
SQANDITLAKMLACSLNYQEIFRHE

Download         Length: 318 bp

>T88620 NZ_CP024819:c1020291-1019974 [Citrobacter freundii]
ATCATGGAAATTCTTCAGACAATGGTTTTCCAGCGTTGGGAGCACAACCTACGGGACAGGAGAGCCAAAACGCTCATTGC
TACACGCCTTTTTCGTTTAGCGAATGGTTTAGCTGGTGATGTTAAACCTGTTGGAGAAGGCGTCAGCGAAATGCGCATTT
CATACGGTCCTGGTTACAGGATTTACTTTAAACAGCAAGGCTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTCGCCTGTTCACTGAATTATCAGGAGATCTTCCGCCATGAATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 9827.36 Da        Isoelectric Point: 6.2975

>AT88620 WP_100193830.1 NZ_CP024819:c1019981-1019703 [Citrobacter freundii]
MNKLTQYDPATALVDDEEIAVFMADALETGDATYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKALGIGFTLKK

Download         Length: 279 bp

>AT88620 NZ_CP024819:c1019981-1019703 [Citrobacter freundii]
ATGAATAAACTTACTCAGTACGATCCCGCCACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGCGATGCAACGTACATTGCAAAAGCACTGGGCGTGGTGGCTCGCGCTAAGGGAATGGCGAACATTGCGGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGAAACCCGACGCTGAAAACCACGCTGGCTGTG
ATGAAGGCGCTCGGTATTGGATTTACACTTAAAAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References