Detailed information of TA system TA088618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 410846..411477
• Replicon: chromosome
• Accession: NZ_CP024819
• Organism: Citrobacter freundii strain CRCB-101

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0J1MPQ6
• Locus tag: CU079_RS02065
• Protein ID: WP_003837806.1
• Coordinates: 411202..411477 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CU079_RS02060
• Protein ID: WP_087051931.1
• Coordinates: 410846..411205 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU079_RS02030	405941..406606	+	666	WP_019078120.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-
CU079_RS02035	406679..407236	+	558	WP_016154847.1	DcrB family lipoprotein	-
CU079_RS02040	407240..408457	-	1218	WP_100193713.1	MFS transporter	-
CU079_RS02045	408591..409640	+	1050	WP_043018840.1	AI-2E family transporter	-
CU079_RS02050	409703..410281	+	579	WP_100193714.1	4'-phosphopantetheinyl transferase AcpT	-
CU079_RS02055	410331..410732	+	402	WP_043018621.1	nickel-responsive transcriptional regulator NikR	-
CU079_RS02060	410846..411205	-	360	WP_087051931.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CU079_RS02065	411202..411477	-	276	WP_003837806.1	type II toxin-antitoxin system HicA family toxin	Toxin
CU079_RS02070	411555..412679	-	1125	WP_075145524.1	ABC transporter permease	-
CU079_RS02075	412679..415426	-	2748	WP_100193715.1	ribosome-associated ATPase/putative transporter RbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10210.88 Da
• Isoelectric Point: 10.7228

>T88618 WP_003837806.1 NZ_CP024819:c411477-411202 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T88618 NZ_CP024819:c411477-411202 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCACGGTGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13184.96 Da
• Isoelectric Point: 4.5348

>AT88618 WP_087051931.1 NZ_CP024819:c411205-410846 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLRDYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT88618 NZ_CP024819:c411205-410846 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGAG
AGATCTCCTTACGAGATTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACA
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Structures

Toxin

• AlphaFold DB: A0A0J1MPQ6