Detailed information of TA system TA088617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 18377..18978
• Replicon: plasmid pCREC-629_2
• Accession: NZ_CP024817
• Organism: Escherichia coli strain CREC-629

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: CU080_RS27030
• Protein ID: WP_001216045.1
• Coordinates: 18598..18978 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CU080_RS27025
• Protein ID: WP_001190712.1
• Coordinates: 18377..18598 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU080_RS26975	13563..13826	+	264	WP_000224220.1	hypothetical protein	-
CU080_RS26980	13837..14436	+	600	WP_053903357.1	DUF551 domain-containing protein	-
CU080_RS26985	14519..14752	+	234	WP_000516537.1	hypothetical protein	-
CU080_RS26990	14931..15224	+	294	WP_001677496.1	hypothetical protein	-
CU080_RS26995	15231..15605	+	375	WP_023154376.1	hypothetical protein	-
CU080_RS27000	15587..16519	+	933	WP_077780118.1	hypothetical protein	-
CU080_RS27005	16516..16878	+	363	WP_001261544.1	hypothetical protein	-
CU080_RS28405	16953..17090	-	138	WP_000123562.1	hypothetical protein	-
CU080_RS27015	17540..17791	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
CU080_RS27020	17915..18304	+	390	WP_000506726.1	DNA repair protein	-
CU080_RS27025	18377..18598	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CU080_RS27030	18598..18978	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CU080_RS27035	18983..19162	+	180	WP_000113019.1	hypothetical protein	-
CU080_RS27040	19190..20233	+	1044	WP_000648823.1	DUF968 domain-containing protein	-
CU080_RS27045	20322..20774	+	453	WP_001369802.1	hypothetical protein	-
CU080_RS27050	20861..22054	+	1194	WP_000219605.1	terminase	-
CU080_RS27055	22054..23538	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96990	96990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T88617 WP_001216045.1 NZ_CP024817:18598-18978 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T88617 NZ_CP024817:18598-18978 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT88617 WP_001190712.1 NZ_CP024817:18377-18598 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT88617 NZ_CP024817:18377-18598 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6