Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 69650..70251 Replicon plasmid pCREC-629_1
Accession NZ_CP024816
Organism Escherichia coli strain CREC-629

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag CU080_RS26180 Protein ID WP_001216034.1
Coordinates 69650..70030 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CU080_RS26185 Protein ID WP_001190712.1
Coordinates 70030..70251 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU080_RS26145 64701..65633 - 933 WP_000081352.1 homocysteine S-methyltransferase -
CU080_RS26150 65620..67023 - 1404 WP_001373486.1 S-methylmethionine permease -
CU080_RS26155 67267..68247 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
CU080_RS26160 68475..68792 + 318 WP_001513659.1 hypothetical protein -
CU080_RS26165 68742..69155 - 414 Protein_89 DDE-type integrase/transposase/recombinase -
CU080_RS26170 69079..69438 - 360 WP_001513660.1 hypothetical protein -
CU080_RS26175 69466..69645 - 180 WP_001513661.1 hypothetical protein -
CU080_RS26180 69650..70030 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CU080_RS26185 70030..70251 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CU080_RS26190 70434..71990 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
CU080_RS26195 71987..73258 + 1272 WP_048266692.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IId / tet(B) / erm(B) / mph(A) / sul1 / qacE / aadA5 / dfrA17 / blaTEM-1B - 1..176274 176274


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T88608 WP_001216034.1 NZ_CP024816:c70030-69650 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T88608 NZ_CP024816:c70030-69650 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT88608 WP_001190712.1 NZ_CP024816:c70251-70030 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT88608 NZ_CP024816:c70251-70030 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References