Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 54948..55507 Replicon plasmid pCREC-629_1
Accession NZ_CP024816
Organism Escherichia coli strain CREC-629

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CU080_RS26055 Protein ID WP_000421272.1
Coordinates 55232..55507 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CU080_RS26050 Protein ID WP_001387467.1
Coordinates 54948..55232 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU080_RS28390 50675..50932 + 258 Protein_66 DUF3330 domain-containing protein -
CU080_RS26025 50983..51687 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
CU080_RS26030 51753..52142 + 390 Protein_68 dihydrodipicolinate synthase family protein -
CU080_RS26035 52167..52547 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
CU080_RS26040 52580..53545 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CU080_RS26045 53591..54343 - 753 WP_000562172.1 IclR family transcriptional regulator -
CU080_RS26050 54948..55232 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CU080_RS26055 55232..55507 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CU080_RS26060 55613..55906 + 294 WP_001105060.1 hypothetical protein -
CU080_RS26065 55941..56069 - 129 Protein_75 IS481 family transposase -
CU080_RS26070 56102..57271 - 1170 WP_001072355.1 hypothetical protein -
CU080_RS26080 57764..58071 + 308 Protein_77 type II toxin-antitoxin system ParD family antitoxin -
CU080_RS26090 58414..58596 + 183 WP_042065278.1 hypothetical protein -
CU080_RS26095 58717..59457 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IId / tet(B) / erm(B) / mph(A) / sul1 / qacE / aadA5 / dfrA17 / blaTEM-1B - 1..176274 176274


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T88607 WP_000421272.1 NZ_CP024816:55232-55507 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T88607 NZ_CP024816:55232-55507 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT88607 WP_001387467.1 NZ_CP024816:54948-55232 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT88607 NZ_CP024816:54948-55232 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References