Detailed information of TA system TA088603

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4455358..4456052
• Replicon: chromosome
• Accession: NZ_CP024815
• Organism: Escherichia coli strain CREC-629

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: CU080_RS23175
• Protein ID: WP_001094398.1
• Coordinates: 4455358..4455726 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CU080_RS23180
• Protein ID: WP_077249034.1
• Coordinates: 4455747..4456052 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU080_RS23155	4450531..4451487	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
CU080_RS23160	4451484..4453682	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
CU080_RS23165	4453692..4454648	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
CU080_RS23170	4454699..4455037	+	339	Protein_4193	LysR family transcriptional regulator	-
CU080_RS23175	4455358..4455726	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
CU080_RS23180	4455747..4456052	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CU080_RS23185	4456156..4456800	-	645	WP_000086755.1	hypothetical protein	-
CU080_RS23190	4456819..4457040	-	222	WP_000692345.1	DUF987 domain-containing protein	-
CU080_RS23195	4457109..4457585	-	477	WP_001424026.1	RadC family protein	-
CU080_RS23200	4457601..4458074	-	474	WP_000855059.1	antirestriction protein	-
CU080_RS23210	4458416..4459237	-	822	WP_001234565.1	DUF945 domain-containing protein	-
CU080_RS28100	4459338..4459546	-	209	Protein_4201	DUF905 domain-containing protein	-
CU080_RS23220	4459647..4460102	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4422851..4456800	33949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T88603 WP_001094398.1 NZ_CP024815:c4455726-4455358 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T88603 NZ_CP024815:c4455726-4455358 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT88603 WP_077249034.1 NZ_CP024815:c4456052-4455747 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT88603 NZ_CP024815:c4456052-4455747 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4