Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3027386..3027912 Replicon chromosome
Accession NZ_CP024815
Organism Escherichia coli strain CREC-629

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CU080_RS15585 Protein ID WP_000323025.1
Coordinates 3027625..3027912 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CU080_RS15580 Protein ID WP_000534858.1
Coordinates 3027386..3027625 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU080_RS15540 3023114..3023347 - 234 WP_001341330.1 IS66 family insertion sequence element accessory protein TnpB -
CU080_RS15545 3023395..3023493 - 99 Protein_2820 IS66 family insertion sequence element accessory protein TnpB -
CU080_RS28245 3023715..3024002 + 288 Protein_2821 hypothetical protein -
CU080_RS15560 3024771..3026084 + 1314 WP_000589005.1 ISNCY family transposase -
CU080_RS15570 3026521..3026853 - 333 WP_001301033.1 protein FlxA -
CU080_RS15575 3027056..3027361 - 306 WP_001326990.1 hypothetical protein -
CU080_RS15580 3027386..3027625 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CU080_RS15585 3027625..3027912 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CU080_RS15590 3027984..3028139 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CU080_RS15595 3028356..3028607 + 252 WP_000980994.1 hypothetical protein -
CU080_RS15600 3028674..3028952 + 279 WP_012304870.1 hypothetical protein -
CU080_RS15605 3028954..3030003 + 1050 WP_001265199.1 DUF968 domain-containing protein -
CU080_RS15610 3030017..3030769 + 753 WP_001047135.1 antitermination protein -
CU080_RS27945 3031047..3031136 - 90 WP_120795389.1 hypothetical protein -
CU080_RS15615 3031191..3031403 - 213 WP_000087756.1 cold shock-like protein CspF -
CU080_RS15620 3031704..3031919 + 216 WP_000066484.1 cold shock-like protein CspB -
CU080_RS28510 3032283..3032453 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
CU080_RS15625 3032673..3032888 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3016471..3049997 33526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T88599 WP_000323025.1 NZ_CP024815:3027625-3027912 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T88599 NZ_CP024815:3027625-3027912 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT88599 WP_000534858.1 NZ_CP024815:3027386-3027625 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT88599 NZ_CP024815:3027386-3027625 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References