Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4506821..4507441 Replicon chromosome
Accession NZ_CP024812
Organism Enterobacter sp. CRENT-193

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag CU081_RS22465 Protein ID WP_015571250.1
Coordinates 4507223..4507441 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag CU081_RS22460 Protein ID WP_006809850.1
Coordinates 4506821..4507195 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU081_RS22450 4501948..4503141 + 1194 WP_058647476.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CU081_RS22455 4503164..4506310 + 3147 WP_022650497.1 multidrug efflux RND transporter permease subunit -
CU081_RS22460 4506821..4507195 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
CU081_RS22465 4507223..4507441 + 219 WP_015571250.1 hemolysin expression modulator Hha Toxin
CU081_RS22470 4507650..4508201 + 552 WP_022650496.1 maltose O-acetyltransferase -
CU081_RS22475 4508318..4508785 + 468 WP_022650495.1 hypothetical protein -
CU081_RS22480 4508757..4510217 - 1461 WP_022650494.1 PLP-dependent aminotransferase family protein -
CU081_RS22485 4510319..4511029 + 711 WP_022650493.1 GNAT family N-acetyltransferase -
CU081_RS22490 4511026..4511166 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
CU081_RS22495 4511169..4511429 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T88579 WP_015571250.1 NZ_CP024812:4507223-4507441 [Enterobacter sp. CRENT-193]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T88579 NZ_CP024812:4507223-4507441 [Enterobacter sp. CRENT-193]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAAAAAAATAAATACGAGCTCTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTGGCTGAAC
TAACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT88579 WP_006809850.1 NZ_CP024812:4506821-4507195 [Enterobacter sp. CRENT-193]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT88579 NZ_CP024812:4506821-4507195 [Enterobacter sp. CRENT-193]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGCCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References