Detailed information of TA system TA088565

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 153274..153977
• Replicon: chromosome
• Accession: NZ_CP024812
• Organism: Enterobacter sp. CRENT-193

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8B5ZZ18
• Locus tag: CU081_RS00780
• Protein ID: WP_039268718.1
• Coordinates: 153274..153615 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CU081_RS00785
• Protein ID: WP_100185454.1
• Coordinates: 153636..153977 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU081_RS00760	149800..150171	+	372	WP_022650323.1	hypothetical protein	-
CU081_RS00765	150229..150696	-	468	WP_022650322.1	SymE family type I addiction module toxin	-
CU081_RS00770	150996..151832	+	837	Protein_145	HNH endonuclease	-
CU081_RS00775	152012..153019	-	1008	WP_039268719.1	restriction endonuclease	-
CU081_RS00780	153274..153615	-	342	WP_039268718.1	TA system toxin CbtA family protein	Toxin
CU081_RS00785	153636..153977	-	342	WP_100185454.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CU081_RS00790	153988..154530	-	543	WP_045259572.1	DNA repair protein RadC	-
CU081_RS00795	154543..154983	-	441	WP_039268716.1	antirestriction protein	-
CU081_RS00800	155014..155835	-	822	WP_039268715.1	DUF945 domain-containing protein	-
CU081_RS00805	155956..156429	-	474	WP_039268714.1	hypothetical protein	-
CU081_RS00810	156501..156953	-	453	WP_039269148.1	hypothetical protein	-
CU081_RS00815	156989..157705	-	717	WP_039268713.1	WYL domain-containing protein	-
CU081_RS00820	157949..158824	-	876	WP_039268712.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	147596..200533	52937

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12772.83 Da
• Isoelectric Point: 9.6548

>T88565 WP_039268718.1 NZ_CP024812:c153615-153274 [Enterobacter sp. CRENT-193]
MKTLPATTPQAAKLCLLPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T88565 NZ_CP024812:c153615-153274 [Enterobacter sp. CRENT-193]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTTGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCTTATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12757.46 Da
• Isoelectric Point: 6.9460

>AT88565 WP_100185454.1 NZ_CP024812:c153977-153636 [Enterobacter sp. CRENT-193]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRVSITGKFSEAECLKLDVAFPHFISQMESMLTTGGMNPRH
AHCVTLYHNGFTCEVDTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT88565 NZ_CP024812:c153977-153636 [Enterobacter sp. CRENT-193]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTAGCTGACCGGGTCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGGAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGTCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A8B5ZZ18