Detailed information of TA system TA088531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1347030..1347655
• Replicon: chromosome
• Accession: NZ_CP024801
• Organism: Escherichia coli strain AMA1167

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CUC42_RS06995
• Protein ID: WP_000911330.1
• Coordinates: 1347257..1347655 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CUC42_RS06990
• Protein ID: WP_000450524.1
• Coordinates: 1347030..1347257 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC42_RS06965	1342833..1343303	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CUC42_RS06970	1343303..1343875	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CUC42_RS06975	1344021..1344899	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CUC42_RS06980	1344916..1345950	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CUC42_RS06985	1346163..1346876	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CUC42_RS06990	1347030..1347257	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CUC42_RS06995	1347257..1347655	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CUC42_RS07000	1347802..1348665	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
CUC42_RS07005	1348680..1350695	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
CUC42_RS07010	1350769..1351110	+	342	Protein_1282	esterase	-
CUC42_RS07015	1351192..1351938	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T88531 WP_000911330.1 NZ_CP024801:1347257-1347655 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T88531 NZ_CP024801:1347257-1347655 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT88531 WP_000450524.1 NZ_CP024801:1347030-1347257 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT88531 NZ_CP024801:1347030-1347257 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33