Detailed information of TA system TA088522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 544565..545202
• Replicon: chromosome
• Accession: NZ_CP024797
• Organism: Bacillus velezensis strain TJ02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CUB85_RS02905
• Protein ID: WP_003156187.1
• Coordinates: 544852..545202 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CUB85_RS02900
• Protein ID: WP_003156188.1
• Coordinates: 544565..544846 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB85_RS02880	540930..541529	-	600	WP_015239195.1	rhomboid family intramembrane serine protease	-
CUB85_RS02885	541622..541987	+	366	WP_094031521.1	holo-ACP synthase	-
CUB85_RS02890	542152..543159	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
CUB85_RS02895	543276..544445	+	1170	WP_025284272.1	alanine racemase	-
CUB85_RS02900	544565..544846	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CUB85_RS02905	544852..545202	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CUB85_RS02910	545320..546141	+	822	WP_003156182.1	STAS domain-containing protein	-
CUB85_RS02915	546146..546511	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CUB85_RS02920	546514..546915	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CUB85_RS02925	546927..547934	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
CUB85_RS02930	547998..548327	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CUB85_RS02935	548324..548806	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CUB85_RS02940	548772..549560	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CUB85_RS02945	549560..550162	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T88522 WP_003156187.1 NZ_CP024797:544852-545202 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T88522 NZ_CP024797:544852-545202 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT88522 WP_003156188.1 NZ_CP024797:544565-544846 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT88522 NZ_CP024797:544565-544846 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5