Detailed information of TA system TA088521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 5149951..5150589
• Replicon: chromosome
• Accession: NZ_CP024795
• Organism: Paenibacillus polymyxa ATCC 842

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: CUU60_RS23055
• Protein ID: WP_007429335.1
• Coordinates: 5150239..5150589 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6CFY0
• Locus tag: CUU60_RS23050
• Protein ID: WP_007429334.1
• Coordinates: 5149951..5150232 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUU60_RS23035 (CUU60_22940)	5146172..5146804	+	633	WP_019686557.1	LysE family transporter	-
CUU60_RS23040 (CUU60_22945)	5146915..5148192	+	1278	WP_019686558.1	outer membrane lipoprotein-sorting protein	-
CUU60_RS23045 (CUU60_22950)	5148461..5149657	+	1197	WP_029514916.1	alanine racemase	-
CUU60_RS23050 (CUU60_22955)	5149951..5150232	+	282	WP_007429334.1	ribbon-helix-helix protein, CopG family	Antitoxin
CUU60_RS23055 (CUU60_22960)	5150239..5150589	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CUU60_RS23060 (CUU60_22965)	5150768..5152990	+	2223	WP_029514917.1	Tex family protein	-
CUU60_RS23065 (CUU60_22970)	5153094..5153228	-	135	WP_016821163.1	cortex morphogenetic protein CmpA	-
CUU60_RS23070 (CUU60_22975)	5153371..5153883	+	513	WP_016821162.1	SprT family protein	-
CUU60_RS23085 (CUU60_22990)	5154262..5155137	-	876	WP_019686561.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T88521 WP_007429335.1 NZ_CP024795:5150239-5150589 [Paenibacillus polymyxa ATCC 842]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T88521 NZ_CP024795:5150239-5150589 [Paenibacillus polymyxa ATCC 842]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACTGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACAATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGACTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10636.25 Da
• Isoelectric Point: 8.5257

>AT88521 WP_007429334.1 NZ_CP024795:5149951-5150232 [Paenibacillus polymyxa ATCC 842]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSGV

Nucleotide

• Length: 282 bp

>AT88521 NZ_CP024795:5149951-5150232 [Paenibacillus polymyxa ATCC 842]
TTGGCCAATTTGCAGAACACCAAAAGAATAATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGC
TATGGAAAATTCCAACCGCAGTGAATTTATCAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTC
GTGAAGCTATGCAGCGTGGATACATGGAAATGGCTAAAATTAACCTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAA
GATGCAAACAGCACCTTGGGCCGTACAGTTAGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1

Antitoxin

• AlphaFold DB: H6CFY0