Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4219750..4220390 Replicon chromosome
Accession NZ_CP024780
Organism Pectobacterium brasiliense strain BZA12

Toxin (Protein)


Gene name vagD Uniprot ID A0A7V8Q162
Locus tag CTV95_RS19140 Protein ID WP_039278477.1
Coordinates 4219974..4220390 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A086EF97
Locus tag CTV95_RS19135 Protein ID WP_039278475.1
Coordinates 4219750..4219977 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTV95_RS19115 4215233..4215730 - 498 WP_039278469.1 M48 family metallopeptidase -
CTV95_RS19120 4215814..4217007 - 1194 WP_100018208.1 aspartate/tyrosine/aromatic aminotransferase -
CTV95_RS19125 4217072..4217686 - 615 WP_014916569.1 YitT family protein -
CTV95_RS19130 4217937..4218416 - 480 WP_010295086.1 Lrp/AsnC family transcriptional regulator -
CTV95_RS19135 4219750..4219977 + 228 WP_039278475.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CTV95_RS19140 4219974..4220390 + 417 WP_039278477.1 type II toxin-antitoxin system VapC family toxin Toxin
CTV95_RS19145 4220469..4221107 - 639 WP_014916568.1 hypothetical protein -
CTV95_RS19150 4221100..4222182 - 1083 WP_100018209.1 alanine racemase -
CTV95_RS19155 4222224..4223630 - 1407 WP_010279223.1 replicative DNA helicase -
CTV95_RS19160 4223758..4224744 + 987 WP_100018210.1 quinone oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15274.80 Da        Isoelectric Point: 8.2931

>T88510 WP_039278477.1 NZ_CP024780:4219974-4220390 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Download         Length: 417 bp

>T88510 NZ_CP024780:4219974-4220390 [Pectobacterium brasiliense]
GTGAGTCGCTTGTTCATGCTGGACACCAATATCTGTTCCTTCATTATGCGGGAACACCCTGCGGAGGTGATTGAAAAGTT
GCAGCAATGCACGTTGCGGCGTGATCGTATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGCGGTTGGTA
AAAAAGCCTCACCACGCCATATGCAACTGGTCGATGCTTTTTGCCGTCGGCTCGATGCGGTATTACCTTGGGATAGCGCC
GCAGTAGATGCCACCACAAAAATCAAAATCGAGCTAGCCGCCGCAGGCACACCTATTGGTCCAAACGATACGGCCATAGC
GGGCCATGCCATCAGCGCTAAGGCCATATTAGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGTTAAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8879.08 Da        Isoelectric Point: 4.8359

>AT88510 WP_039278475.1 NZ_CP024780:4219750-4219977 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Download         Length: 228 bp

>AT88510 NZ_CP024780:4219750-4219977 [Pectobacterium brasiliense]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGCTTGCCGCGTGATATGGACTTTGAAGGGGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATGCTGCGTCCTGTTAAACCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCTGACTTTATGACAGAACGTCAGGATGTGGTCGAGGAGGGGAGATTCAAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8Q162

Antitoxin

Source ID Structure
AlphaFold DB A0A086EF97

References