Detailed information of TA system TA088509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3540737..3541371
• Replicon: chromosome
• Accession: NZ_CP024780
• Organism: Pectobacterium brasiliense strain BZA12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CTV95_RS16020
• Protein ID: WP_080162911.1
• Coordinates: 3540737..3541012 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: CTV95_RS16025
• Protein ID: WP_014917022.1
• Coordinates: 3541009..3541371 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTV95_RS16005	3537756..3539126	-	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-
CTV95_RS16010	3539123..3539821	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
CTV95_RS16015	3539975..3540499	+	525	WP_014917024.1	cell-envelope stress modulator CpxP	-
CTV95_RS16020	3540737..3541012	+	276	WP_080162911.1	type II toxin-antitoxin system HicA family toxin	Toxin
CTV95_RS16025	3541009..3541371	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CTV95_RS16030	3541745..3542932	+	1188	WP_014917021.1	sugar transporter	-
CTV95_RS16035	3543015..3543917	+	903	WP_100017943.1	CDF family cation-efflux transporter FieF	-
CTV95_RS16040	3544125..3545087	+	963	WP_010306854.1	6-phosphofructokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10024.61 Da
• Isoelectric Point: 11.1875

>T88509 WP_080162911.1 NZ_CP024780:3540737-3541012 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLSQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T88509 NZ_CP024780:3540737-3541012 [Pectobacterium brasiliense]
ATGCAGGGAAAGATAGCCAGCCTACGAAAGAAGCAGCGGGAAACGCTAAGCCAAATTTTCAAAACACCAGTACTTTCAGG
TGTTAAATGGTCGAATATTGAGTCACTTGTTACTGCGTTAGGTGGTGAAATTAAGGAAGGTAGCGGGTCACGAGTGCGAT
TTTTATTGAACGGGAGTATCGCCCGATTCCATCGTCCTCATCCGTCACCGGATACTGATAAAGGCGCACTGGTTAGCCTG
CGTGAATGGCTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT88509 WP_014917022.1 NZ_CP024780:3541009-3541371 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT88509 NZ_CP024780:3541009-3541371 [Pectobacterium brasiliense]
ATGACGAAAGCACTCAACACACCAAATACGATGGTTGTTTCTGGACAACCGGCCATTATCAGTTATGTACCGGAAATGGG
TATGTTTCGCGGTAAGTTTATTGGTTTATCTGGCTACTGTGATTTTGTCGCCGACAGTATTAATGGCTTGAGAAGCGAGG
GTGAAATTTCGCTACGCGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAGCGTGAAGAGAAGGTGAAA
ACATTCACCCTCCGTTATCCTGAATCATTCGGGGAACGACTGACCATTGCGGCCGCTGAACGCCAAATTTCAGTGAATAC
GTTTATTGTTGAAACACTTAGCGAACACCTGAAACAGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7