Detailed information of TA system TA088503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2685021..2685547
• Replicon: chromosome
• Accession: NZ_CP024780
• Organism: Pectobacterium brasiliense strain BZA12

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CTV95_RS12180
• Protein ID: WP_100017630.1
• Coordinates: 2685242..2685547 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: CTV95_RS12175
• Protein ID: WP_015855076.1
• Coordinates: 2685021..2685239 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTV95_RS12150	2681226..2682485	+	1260	WP_100017626.1	integrase arm-type DNA-binding domain-containing protein	-
CTV95_RS12155	2682591..2682785	-	195	WP_010299634.1	DUF3950 domain-containing protein	-
CTV95_RS12160	2682971..2683312	+	342	WP_100017627.1	hypothetical protein	-
CTV95_RS12170	2684219..2684962	+	744	WP_100017629.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
CTV95_RS12175	2685021..2685239	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CTV95_RS12180	2685242..2685547	+	306	WP_100017630.1	type II toxin-antitoxin system toxin CcdB	Toxin
CTV95_RS12185	2685978..2687627	+	1650	WP_100017631.1	site-specific DNA-methyltransferase	-
CTV95_RS12190	2687618..2690251	+	2634	WP_100017632.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2681048..2697561	16513

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11745.65 Da
• Isoelectric Point: 4.8500

>T88503 WP_100017630.1 NZ_CP024780:2685242-2685547 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVI
ADLGDYADEIKDALNLMFWGI

Nucleotide

• Length: 306 bp

>T88503 NZ_CP024780:2685242-2685547 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTACAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATTCCGCTTATCGAAGCACATCATCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCGCTGA
TTCGTATCGATGGGGAAGATTATCGGCTGATGACAACTGAGTTGTCGAGCGTTCCGGTTGAGGTCATTGGTGAAGTGATT
GCGGATCTTGGCGACTACGCCGATGAGATTAAGGATGCTCTTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT88503 WP_015855076.1 NZ_CP024780:2685021-2685239 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT88503 NZ_CP024780:2685021-2685239 [Pectobacterium brasiliense]
ATGAAACACCGCGTTAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCTGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGCATAAAGGCTGAAGAGTGGAAGAAAGAAAACCGCGAAGGGATGGAGG
AAGTCGCCCGATTTATCGCACAGAATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46