Detailed information of TA system TA088500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2250043..2250569
• Replicon: chromosome
• Accession: NZ_CP024780
• Organism: Pectobacterium brasiliense strain BZA12

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CTV95_RS10235
• Protein ID: WP_100017463.1
• Coordinates: 2250264..2250569 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A5B8IBF7
• Locus tag: CTV95_RS10230
• Protein ID: WP_038903500.1
• Coordinates: 2250043..2250261 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTV95_RS10205	2246248..2247507	+	1260	WP_100017459.1	integrase arm-type DNA-binding domain-containing protein	-
CTV95_RS10210	2247613..2247807	-	195	WP_010299634.1	DUF3950 domain-containing protein	-
CTV95_RS10215	2247993..2248334	+	342	WP_100017460.1	hypothetical protein	-
CTV95_RS10225	2249241..2249984	+	744	WP_100017462.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
CTV95_RS10230	2250043..2250261	+	219	WP_038903500.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CTV95_RS10235	2250264..2250569	+	306	WP_100017463.1	type II toxin-antitoxin system toxin CcdB	Toxin
CTV95_RS10240	2250676..2251725	-	1050	WP_100017464.1	hypothetical protein	-
CTV95_RS10245	2251734..2252264	-	531	WP_100017465.1	response regulator	-
CTV95_RS10250	2252549..2253037	-	489	WP_100017466.1	GNAT family N-acetyltransferase	-
CTV95_RS10255	2253034..2253327	-	294	WP_100017467.1	DUF1778 domain-containing protein	-
CTV95_RS10260	2253723..2253848	-	126	WP_100017468.1	helix-turn-helix domain-containing protein	-
CTV95_RS10265	2253819..2254064	+	246	WP_100017469.1	type II toxin-antitoxin system antitoxin CcdA	-
CTV95_RS10275	2254185..2254478	-	294	WP_100017470.1	DUF1778 domain-containing protein	-
CTV95_RS10280	2254683..2255183	-	501	WP_014914371.1	serine acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2246162..2268740	22578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11752.64 Da
• Isoelectric Point: 5.6210

>T88500 WP_100017463.1 NZ_CP024780:2250264-2250569 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETSKRRMVIPLIESHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVKVIGETI
ADLGDYADEIKDALNLMFWGI

Nucleotide

• Length: 306 bp

>T88500 NZ_CP024780:2250264-2250569 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGTGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ATCCAAGCGCCGCATGGTCATCCCGCTTATCGAGTCGCATCACCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCGCTGA
TTCGTATCGATGGGGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCGGTTAAGGTGATTGGTGAGACTATC
GCGGATCTTGGCGACTACGCCGATGAGATTAAGGATGCTCTTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.19 Da
• Isoelectric Point: 7.2038

>AT88500 WP_038903500.1 NZ_CP024780:2250043-2250261 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVTRFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT88500 NZ_CP024780:2250043-2250261 [Pectobacterium brasiliense]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTAAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAAGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCACCCGATTTATCGCGCAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5B8IBF7