Detailed information of TA system TA088495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 4587254..4588232
• Replicon: chromosome
• Accession: NZ_CP024771
• Organism: Bacillus thuringiensis LM1212

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CUC43_RS24435
• Protein ID: WP_029438355.1
• Coordinates: 4587495..4588232 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: CUC43_RS24430
• Protein ID: WP_029438356.1
• Coordinates: 4587254..4587382 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC43_RS24415	4583213..4583872	-	660	WP_029438360.1	CatB-related O-acetyltransferase	-
CUC43_RS24420	4584326..4584796	+	471	WP_029438359.1	DUF5065 family protein	-
CUC43_RS24425	4585392..4586861	-	1470	WP_029438358.1	beta-Ala-His dipeptidase	-
CUC43_RS33860	4587005..4587181	-	177	WP_029438357.1	hypothetical protein	-
CUC43_RS24430	4587254..4587382	-	129	WP_029438356.1	hypothetical protein	Antitoxin
CUC43_RS24435	4587495..4588232	-	738	WP_029438355.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CUC43_RS24440	4588421..4589158	-	738	WP_029438354.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CUC43_RS24445	4589325..4589807	-	483	WP_029438353.1	MarR family winged helix-turn-helix transcriptional regulator	-
CUC43_RS24450	4589914..4591599	+	1686	WP_033694540.1	alpha-keto acid decarboxylase family protein	-
CUC43_RS24455	4591647..4592054	-	408	WP_029438351.1	VOC family protein	-
CUC43_RS24460	4592174..4592884	-	711	WP_029438350.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28389.17 Da
• Isoelectric Point: 8.2638

>T88495 WP_029438355.1 NZ_CP024771:c4588232-4587495 [Bacillus thuringiensis LM1212]
VISNIRIGLFILVIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIMIPLEYIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T88495 NZ_CP024771:c4588232-4587495 [Bacillus thuringiensis LM1212]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGTAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCGAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTACGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTATTATGATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5025.42 Da
• Isoelectric Point: 3.9150

>AT88495 WP_029438356.1 NZ_CP024771:c4587382-4587254 [Bacillus thuringiensis LM1212]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT88495 NZ_CP024771:c4587382-4587254 [Bacillus thuringiensis LM1212]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATCATAA