Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1879810..1880452 Replicon chromosome
Accession NZ_CP024771
Organism Bacillus thuringiensis LM1212

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag CUC43_RS10350 Protein ID WP_000635963.1
Coordinates 1880102..1880452 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag CUC43_RS10345 Protein ID WP_000004570.1
Coordinates 1879810..1880097 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC43_RS10320 1874976..1875938 + 963 WP_029439196.1 UV DNA damage repair endonuclease UvsE -
CUC43_RS10325 1876106..1876654 - 549 WP_029439197.1 rhomboid family intramembrane serine protease -
CUC43_RS10330 1876747..1877106 + 360 WP_000635047.1 holo-ACP synthase -
CUC43_RS10335 1877263..1878213 + 951 WP_029141035.1 outer membrane lipoprotein carrier protein LolA -
CUC43_RS10340 1878332..1879501 + 1170 WP_029439198.1 alanine racemase -
CUC43_RS10345 1879810..1880097 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
CUC43_RS10350 1880102..1880452 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CUC43_RS10355 1880520..1882688 + 2169 WP_029439199.1 RNA-binding transcriptional accessory protein -
CUC43_RS10360 1882746..1882862 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
CUC43_RS10365 1883058..1883516 + 459 WP_029439200.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T88494 WP_000635963.1 NZ_CP024771:1880102-1880452 [Bacillus thuringiensis LM1212]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T88494 NZ_CP024771:1880102-1880452 [Bacillus thuringiensis LM1212]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT88494 WP_000004570.1 NZ_CP024771:1879810-1880097 [Bacillus thuringiensis LM1212]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT88494 NZ_CP024771:1879810-1880097 [Bacillus thuringiensis LM1212]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTACCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References