Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 99329..99854 Replicon plasmid p1NQ3
Accession NZ_CP024721
Organism Escherichia coli isolate NQ3

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CUB98_RS27685 Protein ID WP_001159868.1
Coordinates 99329..99634 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CUB98_RS27690 Protein ID WP_000813634.1
Coordinates 99636..99854 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB98_RS27670 95239..96405 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CUB98_RS27675 96993..97748 - 756 WP_000852146.1 replication initiation protein RepE -
CUB98_RS27680 98522..99328 - 807 WP_000016982.1 site-specific integrase -
CUB98_RS27685 99329..99634 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CUB98_RS27690 99636..99854 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CUB98_RS27695 100400..100912 + 513 WP_000151784.1 hypothetical protein -
CUB98_RS27700 100946..102079 - 1134 WP_000545986.1 DUF3800 domain-containing protein -
CUB98_RS27705 102246..103019 - 774 WP_000905949.1 hypothetical protein -
CUB98_RS27710 103032..103532 - 501 WP_000528932.1 hypothetical protein -
CUB98_RS27715 103797..104027 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CUB98_RS27725 104109..104806 + 698 Protein_123 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aadA5 / qacE / sul1 - 1..111776 111776


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T88489 WP_001159868.1 NZ_CP024721:c99634-99329 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T88489 NZ_CP024721:c99634-99329 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT88489 WP_000813634.1 NZ_CP024721:c99854-99636 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT88489 NZ_CP024721:c99854-99636 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References