Detailed information of TA system TA088478

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3544459..3545153
• Replicon: chromosome
• Accession: NZ_CP024720
• Organism: Escherichia coli isolate NQ3

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: CUB98_RS18715
• Protein ID: WP_001094398.1
• Coordinates: 3544459..3544827 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CUB98_RS18720
• Protein ID: WP_077249034.1
• Coordinates: 3544848..3545153 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB98_RS18680	3539824..3541467	+	1644	WP_001265646.1	fimbrial adhesin EcpD	-
CUB98_RS18685	3541436..3542146	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
CUB98_RS18690	3542453..3542782	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
CUB98_RS18695	3542794..3542957	-	164	Protein_3431	tail fiber assembly protein	-
CUB98_RS18700	3543031..3543645	-	615	WP_001019923.1	YagU family protein	-
CUB98_RS18705	3543860..3544045	-	186	WP_000146240.1	hypothetical protein	-
CUB98_RS28240	3544094..3544252	+	159	Protein_3434	integrase	-
CUB98_RS18715	3544459..3544827	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
CUB98_RS18720	3544848..3545153	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CUB98_RS18725	3545257..3545901	-	645	WP_000086755.1	hypothetical protein	-
CUB98_RS18730	3545920..3546141	-	222	WP_000692345.1	DUF987 domain-containing protein	-
CUB98_RS18735	3546210..3546686	-	477	WP_001186773.1	RadC family protein	-
CUB98_RS18740	3546702..3547187	-	486	WP_000214317.1	antirestriction protein	-
CUB98_RS18745	3547278..3548096	-	819	WP_001234702.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3505748..3555309	49561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T88478 WP_001094398.1 NZ_CP024720:c3544827-3544459 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T88478 NZ_CP024720:c3544827-3544459 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT88478 WP_077249034.1 NZ_CP024720:c3545153-3544848 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT88478 NZ_CP024720:c3545153-3544848 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4