Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 23353..23912 Replicon plasmid p1LS4
Accession NZ_CP024718
Organism Escherichia coli strain LS4

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag CUB99_RS27175 Protein ID WP_000421272.1
Coordinates 23353..23628 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CUB99_RS27180 Protein ID WP_001387467.1
Coordinates 23628..23912 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB99_RS27135 19403..20143 - 741 WP_001066953.1 tyrosine-type recombinase/integrase -
CUB99_RS27140 20264..20446 - 183 WP_042344623.1 hypothetical protein -
CUB99_RS27150 20789..21096 - 308 Protein_26 type II toxin-antitoxin system ParD family antitoxin -
CUB99_RS27160 21589..22758 + 1170 WP_001072355.1 hypothetical protein -
CUB99_RS27165 22791..22919 + 129 Protein_28 IS481 family transposase -
CUB99_RS27170 22954..23247 - 294 WP_001105060.1 hypothetical protein -
CUB99_RS27175 23353..23628 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CUB99_RS27180 23628..23912 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CUB99_RS27185 24517..25269 + 753 WP_000562172.1 IclR family transcriptional regulator -
CUB99_RS27190 25315..26280 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
CUB99_RS27195 26313..26693 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
CUB99_RS27200 26718..27608 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
CUB99_RS27210 28009..28449 + 441 Protein_36 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aadA5 / qacE / sul1 - 1..111779 111779


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T88455 WP_000421272.1 NZ_CP024718:c23628-23353 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T88455 NZ_CP024718:c23628-23353 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT88455 WP_001387467.1 NZ_CP024718:c23912-23628 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT88455 NZ_CP024718:c23912-23628 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References