Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 10735..11260 Replicon plasmid p1LS4
Accession NZ_CP024718
Organism Escherichia coli strain LS4

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CUB99_RS27065 Protein ID WP_001159868.1
Coordinates 10735..11040 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CUB99_RS27070 Protein ID WP_000813634.1
Coordinates 11042..11260 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB99_RS27050 6645..7811 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
CUB99_RS27055 8399..9154 - 756 WP_000852146.1 replication initiation protein RepE -
CUB99_RS27060 9928..10734 - 807 WP_000016982.1 site-specific integrase -
CUB99_RS27065 10735..11040 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CUB99_RS27070 11042..11260 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CUB99_RS27075 11806..12318 + 513 WP_000151784.1 hypothetical protein -
CUB99_RS27080 12352..13485 - 1134 WP_000545986.1 DUF3800 domain-containing protein -
CUB99_RS27085 13652..14425 - 774 WP_000905949.1 hypothetical protein -
CUB99_RS27090 14438..14938 - 501 WP_000528932.1 hypothetical protein -
CUB99_RS27095 15203..15433 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CUB99_RS27105 15515..16212 + 698 Protein_19 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aadA5 / qacE / sul1 - 1..111779 111779


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T88454 WP_001159868.1 NZ_CP024718:c11040-10735 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T88454 NZ_CP024718:c11040-10735 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT88454 WP_000813634.1 NZ_CP024718:c11260-11042 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT88454 NZ_CP024718:c11260-11042 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References