Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4455654..4456485 Replicon chromosome
Accession NZ_CP024717
Organism Escherichia coli strain LS4

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag CUB99_RS22990 Protein ID WP_000854815.1
Coordinates 4455654..4456028 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag CUB99_RS22995 Protein ID WP_001280918.1
Coordinates 4456117..4456485 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB99_RS22955 4451050..4452216 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
CUB99_RS22960 4452335..4452808 + 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
CUB99_RS22965 4453006..4454064 + 1059 WP_099996231.1 FUSC family protein -
CUB99_RS22970 4454236..4454565 + 330 WP_000450409.1 DUF496 family protein -
CUB99_RS22975 4454666..4454848 - 183 WP_001296208.1 hypothetical protein -
CUB99_RS22980 4454902..4455048 + 147 Protein_4211 transposase domain-containing protein -
CUB99_RS28020 4455337..4455450 - 114 WP_001161660.1 DUF957 domain-containing protein -
CUB99_RS22985 4455463..4455657 - 195 WP_000988600.1 hypothetical protein -
CUB99_RS22990 4455654..4456028 - 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
CUB99_RS22995 4456117..4456485 - 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CUB99_RS23000 4456501..4457145 - 645 WP_000086752.1 hypothetical protein -
CUB99_RS23005 4457164..4457385 - 222 WP_000692345.1 DUF987 domain-containing protein -
CUB99_RS23010 4457448..4457924 - 477 WP_001186200.1 RadC family protein -
CUB99_RS23015 4457940..4458413 - 474 WP_001542276.1 antirestriction protein -
CUB99_RS23020 4458507..4458752 - 246 WP_001164966.1 hypothetical protein -
CUB99_RS23025 4458752..4459570 - 819 WP_001542275.1 DUF945 domain-containing protein -
CUB99_RS23035 4459791..4460201 - 411 WP_000846703.1 hypothetical protein -
CUB99_RS23040 4460217..4460567 - 351 Protein_4223 hypothetical protein -
CUB99_RS23050 4460650..4461396 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T88444 WP_000854815.1 NZ_CP024717:c4456028-4455654 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T88444 NZ_CP024717:c4456028-4455654 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT88444 WP_001280918.1 NZ_CP024717:c4456485-4456117 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT88444 NZ_CP024717:c4456485-4456117 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References