Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3489549..3490203 Replicon chromosome
Accession NZ_CP024717
Organism Escherichia coli strain LS4

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag CUB99_RS18390 Protein ID WP_000244765.1
Coordinates 3489796..3490203 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag CUB99_RS18385 Protein ID WP_000354050.1
Coordinates 3489549..3489815 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB99_RS18365 3485637..3487070 - 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -
CUB99_RS18370 3487115..3487426 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CUB99_RS18375 3487590..3488249 + 660 WP_000250275.1 hemolysin III family protein -
CUB99_RS18380 3488326..3489306 - 981 WP_000886084.1 tRNA-modifying protein YgfZ -
CUB99_RS18385 3489549..3489815 + 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
CUB99_RS18390 3489796..3490203 + 408 WP_000244765.1 protein YgfX Toxin
CUB99_RS18395 3490243..3490764 - 522 WP_001055874.1 flavodoxin FldB -
CUB99_RS18400 3490876..3491772 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CUB99_RS18405 3491797..3492507 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CUB99_RS18410 3492513..3494246 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T88442 WP_000244765.1 NZ_CP024717:3489796-3490203 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T88442 NZ_CP024717:3489796-3490203 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT88442 WP_000354050.1 NZ_CP024717:3489549-3489815 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88442 NZ_CP024717:3489549-3489815 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References