Detailed information of TA system TA088435

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 1835821..1836522
• Replicon: chromosome
• Accession: NZ_CP024717
• Organism: Escherichia coli strain LS4

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A515RBV3
• Locus tag: CUB99_RS09935
• Protein ID: WP_001531265.1
• Coordinates: 1835821..1836156 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CUB99_RS09940
• Protein ID: WP_032143152.1
• Coordinates: 1836187..1836522 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB99_RS09905	1832760..1832931	-	172	Protein_1817	integrase	-
CUB99_RS09915	1833342..1834139	-	798	WP_001531269.1	helix-turn-helix transcriptional regulator	-
CUB99_RS09920	1834253..1834528	-	276	WP_001531268.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CUB99_RS09925	1834532..1834780	-	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
CUB99_RS09930	1834857..1835690	-	834	WP_001531266.1	DUF4942 domain-containing protein	-
CUB99_RS09935	1835821..1836156	-	336	WP_001531265.1	TA system toxin CbtA family protein	Toxin
CUB99_RS09940	1836187..1836522	-	336	WP_032143152.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CUB99_RS09945	1836522..1836995	-	474	WP_001531261.1	DNA repair protein RadC	-
CUB99_RS09950	1837025..1837843	-	819	WP_001531260.1	DUF945 domain-containing protein	-
CUB99_RS09955	1838079..1839032	-	954	WP_001531259.1	hypothetical protein	-
CUB99_RS09960	1839625..1840239	+	615	WP_001531257.1	inovirus Gp2 family protein	-
CUB99_RS09965	1840357..1840575	+	219	WP_001531256.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (11-110)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12867.89 Da
• Isoelectric Point: 9.8953

>T88435 WP_001531265.1 NZ_CP024717:c1836156-1835821 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRRHNTP

Nucleotide

• Length: 336 bp

>T88435 NZ_CP024717:c1836156-1835821 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACTACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAATATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTACGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCCG
CCATAACACGCCATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12505.48 Da
• Isoelectric Point: 7.1637

>AT88435 WP_032143152.1 NZ_CP024717:c1836522-1836187 [Escherichia coli]
MLQKDKVMQQECGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADDELSACQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT88435 NZ_CP024717:c1836522-1836187 [Escherichia coli]
ATGCTGCAAAAGGATAAGGTCATGCAACAAGAATGTGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGATGAACTCTCCGCCTGCCAACAACGT
ACCGTCACACTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A515RBV3