Detailed information of TA system TA088433

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1298861..1299555
• Replicon: chromosome
• Accession: NZ_CP024717
• Organism: Escherichia coli strain LS4

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: CUB99_RS07140
• Protein ID: WP_001094398.1
• Coordinates: 1298861..1299229 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: CUB99_RS07145
• Protein ID: WP_077249034.1
• Coordinates: 1299250..1299555 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB99_RS07105	1294226..1295869	+	1644	WP_001265646.1	fimbrial adhesin EcpD	-
CUB99_RS07110	1295838..1296548	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
CUB99_RS07115	1296855..1297184	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
CUB99_RS07120	1297196..1297359	-	164	Protein_1307	tail fiber assembly protein	-
CUB99_RS07125	1297433..1298047	-	615	WP_001019923.1	YagU family protein	-
CUB99_RS07130	1298262..1298447	-	186	WP_000146240.1	hypothetical protein	-
CUB99_RS28155	1298496..1298654	+	159	Protein_1310	integrase	-
CUB99_RS07140	1298861..1299229	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
CUB99_RS07145	1299250..1299555	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CUB99_RS07150	1299659..1300303	-	645	WP_000086755.1	hypothetical protein	-
CUB99_RS07155	1300322..1300543	-	222	WP_000692345.1	DUF987 domain-containing protein	-
CUB99_RS07160	1300612..1301088	-	477	WP_001186773.1	RadC family protein	-
CUB99_RS07165	1301104..1301589	-	486	WP_000214317.1	antirestriction protein	-
CUB99_RS07170	1301680..1302498	-	819	WP_001234702.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	1260150..1309711	49561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T88433 WP_001094398.1 NZ_CP024717:c1299229-1298861 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T88433 NZ_CP024717:c1299229-1298861 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT88433 WP_077249034.1 NZ_CP024717:c1299555-1299250 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT88433 NZ_CP024717:c1299555-1299250 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4