Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1117044..1117662 Replicon chromosome
Accession NZ_CP024717
Organism Escherichia coli strain LS4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CUB99_RS06220 Protein ID WP_001291435.1
Coordinates 1117444..1117662 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CUB99_RS06215 Protein ID WP_000344800.1
Coordinates 1117044..1117418 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB99_RS06205 1112133..1113326 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CUB99_RS06210 1113349..1116498 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
CUB99_RS06215 1117044..1117418 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CUB99_RS06220 1117444..1117662 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CUB99_RS06225 1117836..1118387 + 552 WP_000102539.1 maltose O-acetyltransferase -
CUB99_RS06230 1118503..1118973 + 471 WP_000136192.1 YlaC family protein -
CUB99_RS06235 1119137..1120687 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CUB99_RS06240 1120729..1121082 - 354 WP_000878135.1 DUF1428 family protein -
CUB99_RS06250 1121461..1121772 + 312 WP_000409908.1 MGMT family protein -
CUB99_RS06255 1121803..1122375 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T88432 WP_001291435.1 NZ_CP024717:1117444-1117662 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T88432 NZ_CP024717:1117444-1117662 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT88432 WP_000344800.1 NZ_CP024717:1117044-1117418 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT88432 NZ_CP024717:1117044-1117418 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References