Detailed information of TA system TA088429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 243156..243715
• Replicon: chromosome
• Accession: NZ_CP024715
• Organism: Brucella melitensis strain Rev.1 (passage 101)

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q8YH01
• Locus tag: CUC12_RS01345
• Protein ID: WP_004683741.1
• Coordinates: 243470..243715 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: CUC12_RS01340
• Protein ID: WP_004683740.1
• Coordinates: 243156..243470 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC12_RS01290	238861..239064	-	204	WP_004683737.1	hypothetical protein	-
CUC12_RS01295	239067..239282	-	216	WP_005968947.1	hypothetical protein	-
CUC12_RS01300	239279..239482	-	204	WP_002964088.1	hypothetical protein	-
CUC12_RS01305	239532..240236	+	705	WP_005968944.1	hypothetical protein	-
CUC12_RS01310	240233..240463	+	231	WP_002964091.1	hypothetical protein	-
CUC12_RS01315	240460..240735	+	276	WP_002964092.1	hypothetical protein	-
CUC12_RS01320	240758..241345	+	588	WP_004683738.1	hypothetical protein	-
CUC12_RS01325	241580..242290	+	711	WP_004683739.1	porin family protein	-
CUC12_RS01330	242638..242808	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
CUC12_RS01340	243156..243470	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
CUC12_RS01345	243470..243715	-	246	WP_004683741.1	BrnT family toxin	Toxin
CUC12_RS01350	243848..244486	+	639	Protein_237	tRNA-guanine transglycosylase	-
CUC12_RS01360	244556..245313	+	758	WP_196219767.1	IS5 family transposase	-
CUC12_RS01365	245314..246087	+	774	WP_002967634.1	hypothetical protein	-
CUC12_RS01370	246418..247869	+	1452	WP_004683742.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	237089..251185	14096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9196.83 Da
• Isoelectric Point: 10.3158

>T88429 WP_004683741.1 NZ_CP024715:c243715-243470 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVFFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T88429 NZ_CP024715:c243715-243470 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCTTTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT88429 WP_004683740.1 NZ_CP024715:c243470-243156 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT88429 NZ_CP024715:c243470-243156 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: Q8YH01

Antitoxin

• AlphaFold DB: C0RIV1