Detailed information of TA system TA088427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 6053921..6054681
• Replicon: chromosome
• Accession: NZ_CP024712
• Organism: Pseudomonas syringae pv. actinidiae strain MAFF212063

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CT122_RS28700
• Protein ID: WP_099979092.1
• Coordinates: 6053921..6054217 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A2G9KX16
• Locus tag: CT122_RS28705
• Protein ID: WP_005737550.1
• Coordinates: 6054214..6054681 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CT122_RS28680	6049101..6050171	-	1071	WP_099979095.1	UvrD-helicase domain-containing protein	-
CT122_RS28685	6050164..6051765	-	1602	WP_099979094.1	AAA family ATPase	-
CT122_RS28690	6052454..6053650	-	1197	WP_099979093.1	type II toxin-antitoxin system HipA family toxin	-
CT122_RS28695	6053650..6053901	-	252	WP_087675199.1	helix-turn-helix domain-containing protein	-
CT122_RS28700	6053921..6054217	-	297	WP_099979092.1	hypothetical protein	Toxin
CT122_RS28705	6054214..6054681	-	468	WP_005737550.1	helix-turn-helix domain-containing protein	Antitoxin
CT122_RS33325	6054956..6055444	+	489	WP_020307385.1	hypothetical protein	-
CT122_RS28715	6055498..6056091	+	594	WP_020307386.1	hypothetical protein	-
CT122_RS28720	6056246..6056878	-	633	WP_099979091.1	4'-phosphopantetheinyl transferase superfamily protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	6038529..6068823	30294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11554.45 Da
• Isoelectric Point: 9.9082

>T88427 WP_099979092.1 NZ_CP024712:c6054217-6053921 [Pseudomonas syringae pv. actinidiae]
MRHSPFTAFYDANVLYPAPLRDFLMHLALTGVYRARWSSKIHDEWKRNLLINRPELTREQLDRTSALMDKAVPDVTTQMI
CPHINLIFSKVRYTLHSL

Nucleotide

• Length: 297 bp

>T88427 NZ_CP024712:c6054217-6053921 [Pseudomonas syringae pv. actinidiae]
ATGAGGCATTCGCCGTTTACAGCTTTCTATGACGCCAACGTCCTATACCCGGCACCGCTTCGTGACTTTCTGATGCACTT
GGCATTGACTGGCGTGTACCGCGCACGCTGGTCTTCTAAAATTCATGACGAGTGGAAGCGCAACCTTCTAATCAATCGTC
CAGAGCTTACCCGTGAGCAACTGGATCGCACGTCTGCGCTGATGGATAAGGCAGTGCCGGACGTTACGACTCAAATGATC
TGCCCTCACATCAATTTGATTTTCTCAAAGGTCAGGTATACGTTACATTCACTCTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17233.75 Da
• Isoelectric Point: 5.2911

>AT88427 WP_005737550.1 NZ_CP024712:c6054681-6054214 [Pseudomonas syringae pv. actinidiae]
MSSADATRTVLPGEKEIAAAVESSRQLAAFLTTKCDTQRIELVDETQQREVVELPMFALRLLGEILSELALGNAVKVVPI
HAELTTQEAADMLNVSRPHLVKMLDQGMLPHTKTGRHRRVKFADLMSYKQQRDQASREAMDELAAQAQELGMGYE

Nucleotide

• Length: 468 bp

>AT88427 NZ_CP024712:c6054681-6054214 [Pseudomonas syringae pv. actinidiae]
ATGAGCAGCGCAGACGCCACTCGCACCGTTCTTCCTGGGGAGAAAGAAATCGCTGCGGCGGTTGAATCAAGTCGGCAATT
AGCCGCGTTTCTTACAACGAAATGTGACACCCAGCGCATTGAGCTTGTGGACGAAACGCAACAGCGCGAAGTCGTCGAGC
TGCCTATGTTTGCACTCCGTTTGCTCGGCGAAATTCTCAGTGAGCTTGCGCTGGGTAACGCCGTCAAGGTGGTCCCCATT
CATGCTGAACTCACTACTCAGGAAGCAGCAGACATGCTCAATGTGTCCCGGCCTCATCTGGTGAAGATGCTGGACCAAGG
CATGTTGCCGCACACTAAAACCGGCAGGCACCGCCGAGTCAAATTTGCTGATTTGATGAGCTACAAGCAGCAGCGCGACC
AGGCAAGTCGCGAAGCCATGGACGAGTTGGCCGCGCAAGCCCAAGAACTAGGGATGGGATATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2G9KX16