Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 189300..190210 Replicon plasmid pPUTH1
Accession NZ_CP024708
Organism Klebsiella pneumoniae strain cr-hvkp3

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag CTM43_RS27455 Protein ID WP_004026354.1
Coordinates 189300..189770 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag CTM43_RS27460 Protein ID WP_004026357.1
Coordinates 189767..190210 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTM43_RS28980 184662..184823 + 162 WP_164507110.1 hypothetical protein -
CTM43_RS28985 184940..185104 + 165 WP_011154586.1 hypothetical protein -
CTM43_RS27430 185180..185422 + 243 WP_071826037.1 hypothetical protein -
CTM43_RS27435 185801..185959 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
CTM43_RS27440 186031..187212 + 1182 WP_004883380.1 IS481 family transposase -
CTM43_RS27445 187783..188043 + 261 WP_004026352.1 hypothetical protein -
CTM43_RS27450 188639..188845 + 207 WP_004902422.1 hypothetical protein -
CTM43_RS27455 189300..189770 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
CTM43_RS27460 189767..190210 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
CTM43_RS27465 190311..190814 - 504 Protein_207 DUF4113 domain-containing protein -
CTM43_RS27470 190912..191133 - 222 WP_004213643.1 DUF2188 domain-containing protein -
CTM43_RS27475 191278..191433 - 156 WP_004213642.1 hypothetical protein -
CTM43_RS27480 191508..193634 - 2127 WP_029884227.1 TonB-dependent receptor family protein -
CTM43_RS27485 193721..194674 - 954 Protein_211 fec operon regulator FecR -
CTM43_RS27490 194671..195192 - 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA 1..237216 237216
- flank IS/Tn - - 186031..187212 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T88383 WP_004026354.1 NZ_CP024708:c189770-189300 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T88383 NZ_CP024708:c189770-189300 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT88383 WP_004026357.1 NZ_CP024708:c190210-189767 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT88383 NZ_CP024708:c190210-189767 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References