Detailed information of TA system TA088381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 70614..71284
• Replicon: plasmid pPUTH1
• Accession: NZ_CP024708
• Organism: Klebsiella pneumoniae strain cr-hvkp3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: CTM43_RS26790
• Protein ID: WP_004213072.1
• Coordinates: 70841..71284 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: CTM43_RS26785
• Protein ID: WP_004213073.1
• Coordinates: 70614..70844 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTM43_RS26760	66837..67736	-	900	WP_004225022.1	nucleotide-binding protein	-
CTM43_RS26765	67726..68016	-	291	WP_004213078.1	hypothetical protein	-
CTM43_RS26770	68368..68574	+	207	WP_004213077.1	hypothetical protein	-
CTM43_RS26775	68564..68857	-	294	WP_004213076.1	hypothetical protein	-
CTM43_RS26780	68873..70006	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
CTM43_RS26785	70614..70844	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CTM43_RS26790	70841..71284	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
CTM43_RS26795	71298..71714	-	417	Protein_78	restriction endonuclease	-
CTM43_RS26800	71718..72011	-	294	Protein_79	transposase	-
CTM43_RS29065	72428..73066	+	639	Protein_80	response regulator	-
CTM43_RS26820	73581..73987	-	407	Protein_81	GAF domain-containing protein	-
CTM43_RS26830	74078..74998	-	921	WP_029884592.1	DUF1471 domain-containing protein	-
CTM43_RS26835	75047..75538	-	492	WP_011251327.1	DM13 domain-containing protein	-
CTM43_RS26840	75601..75876	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA	1..237216	237216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T88381 WP_004213072.1 NZ_CP024708:70841-71284 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T88381 NZ_CP024708:70841-71284 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT88381 WP_004213073.1 NZ_CP024708:70614-70844 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT88381 NZ_CP024708:70614-70844 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620