Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 261736..262475 Replicon chromosome
Accession NZ_CP024707
Organism Klebsiella pneumoniae strain cr-hvkp3

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag CTM43_RS01320 Protein ID WP_004901283.1
Coordinates 261990..262475 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CTM43_RS01315 Protein ID WP_003026799.1
Coordinates 261736..262002 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTM43_RS01300 257240..259309 + 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -
CTM43_RS01305 259605..260974 + 1370 WP_087661561.1 IS3 family transposase -
CTM43_RS01310 261175..261603 + 429 WP_004901287.1 GFA family protein -
CTM43_RS01315 261736..262002 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CTM43_RS01320 261990..262475 + 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
CTM43_RS01325 262800..263846 - 1047 WP_022615678.1 IS481-like element ISKpn28 family transposase -
CTM43_RS01330 263920..264072 + 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
CTM43_RS01335 264374..265993 + 1620 WP_029884695.1 ATP-binding cassette domain-containing protein -
CTM43_RS01340 266091..266303 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
CTM43_RS01345 266555..266845 - 291 WP_002921931.1 HTH-type transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 260249..263846 3597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T88364 WP_004901283.1 NZ_CP024707:261990-262475 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T88364 NZ_CP024707:261990-262475 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT88364 WP_003026799.1 NZ_CP024707:261736-262002 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT88364 NZ_CP024707:261736-262002 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References