Detailed information of TA system TA088348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4477829..4478495
• Replicon: chromosome
• Accession: NZ_CP024683
• Organism: Citrobacter freundii strain UMH13

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A8B5QBX6
• Locus tag: CUC45_RS21910
• Protein ID: WP_032948292.1
• Coordinates: 4477829..4478188 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: CUC45_RS21915
• Protein ID: WP_003031625.1
• Coordinates: 4478178..4478495 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC45_RS21900	4475229..4476860	+	1632	WP_003031618.1	Na/Pi cotransporter family protein	-
CUC45_RS21905	4476926..4477615	-	690	WP_003841421.1	dipeptidase PepE	-
CUC45_RS21910	4477829..4478188	+	360	WP_032948292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CUC45_RS21915	4478178..4478495	+	318	WP_003031625.1	helix-turn-helix domain-containing protein	Antitoxin
CUC45_RS21920	4478505..4478783	-	279	WP_032948293.1	putative addiction module antidote protein	-
CUC45_RS21925	4479187..4479348	-	162	WP_003841416.1	phage protein	-
CUC45_RS21930	4479490..4479846	+	357	WP_032948294.1	hypothetical protein	-
CUC45_RS21935	4479898..4480710	-	813	WP_032948295.1	shikimate 5-dehydrogenase	-
CUC45_RS21940	4480712..4481935	-	1224	WP_032948296.1	L-sorbose 1-phosphate reductase	-
CUC45_RS21945	4482003..4482827	-	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13461.47 Da
• Isoelectric Point: 10.2555

>T88348 WP_032948292.1 NZ_CP024683:4477829-4478188 [Citrobacter freundii]
MTKPLYWVGHARKDLQGMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNTWRAVYTIQLKNAVYVL
HVFQKKSVAGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T88348 NZ_CP024683:4477829-4478188 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCATGCCAGGAAAGATCTACAGGGAATGCCAGAACATGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGCGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACACATGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTGCCGGGAAGGCAACGCCCAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11522.46 Da
• Isoelectric Point: 9.0005

>AT88348 WP_003031625.1 NZ_CP024683:4478178-4478495 [Citrobacter freundii]
MSHEVEASSGNVYADLGVYDAEQMQLKAQLAMVIGEILQRKGLTQQQSAQLLGMTQPKLSNLLRGQFRGISEVKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT88348 NZ_CP024683:4478178-4478495 [Citrobacter freundii]
ATGTCACATGAAGTTGAAGCCAGTAGCGGAAATGTTTACGCCGATTTAGGCGTGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAACTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGTCCGCGCAGCTACTGGGAA
TGACGCAGCCTAAACTTTCCAACCTGCTACGTGGTCAGTTTCGTGGTATCAGTGAAGTAAAGATGCTCGAATGCCTGGTG
AGACTGGGTCGTGATGTAGAGATTGTTGTTGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A8B5QBX6